Composite

Part:BBa_K4297077

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 06:55, 11 October 2022 by GYang1213 (Talk | contribs)


PL-CBE

The composite part contains Lambda cI , cytidine deaminase, Uracil DNA glycosylase inhibitor and LVA degradation tag. CDA catalyzes the deamination of cytosine in the top (non-complementary) strand located within 15-20 bases upstream of PAM, resulting in a C-T mutation.

Fig. The plasmid construction process

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2097
    Illegal NheI site found at 6019
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5432
    Illegal BamHI site found at 4376
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5111
    Illegal AgeI site found at 5757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown


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Categories
Parameters
None