![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4297077
Designed by: Yang Gu Group: iGEM22_NJXDF-CHN (2022-10-10)
PL-CBE
The composite part contains Lambda cI , cytidine deaminase, Uracil DNA glycosylase inhibitor and LVA degradation tag. CDA catalyzes the deamination of cytosine in the top (non-complementary) strand located within 15-20 bases upstream of PAM, resulting in a C-T mutation.
![](https://static.igem.wiki/teams/4297/wiki/1.png)
- Fig. The plasmid construction process
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2097
Illegal NheI site found at 6019 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5432
Illegal BamHI site found at 4376 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 5111
Illegal AgeI site found at 5757 - 1000INCOMPATIBLE WITH RFC[1000]Unknown
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Categories
Parameters
None |