Coding

Part:BBa_K4252009:Design

Designed by: Yuhan Wu   Group: iGEM22_HNU_China   (2022-09-26)
Revision as of 06:45, 11 October 2022 by WuYuhan (Talk | contribs)

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Phosphate-specific transportor (Pst)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
    Illegal AgeI site found at 720
    Illegal AgeI site found at 2721
    Illegal AgeI site found at 3917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3737


Design Notes

The wild-type E. coli has an inherent regulation mechanism, which means the Pst system will be repressed if the concentration of phosphate is higher than 10-3M and it is much more difficult to find and knockout the regulator genes preciously. But we find that the mainly negative feedback regulation is through the combination between phoB protein and Upstream promoter of the PstS gene. Therefore, the Pst gene we transferred will not be controlled by this way. However, unavoidably, there may be other unknown negative feedback regulations, that is, the overexpression of Pst could minimize the effect caused by them.

Source

Escherichia coli str. K-12 substr. MG1655

References

[1] Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression.
[2] Regulation of the pho regulon of Escherichia coli K-12: Cloning of the regulatory genes phoB and phoR and identification of their gene products.