Regulatory

Part:BBa_K4317001

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-11)
Revision as of 05:46, 11 October 2022 by Envien mc (Talk | contribs)

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P2069M

P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.

P2069M_ref.png


800px-P2069M.png


Usage and Biology

800px-Promoter_strength.png

Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)

References

Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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