Regulatory

Part:BBa_K4317001

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-11)
Revision as of 05:23, 11 October 2022 by Envien mc (Talk | contribs)


P2069M

P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.

P2069M_ref.png Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None