Composite

Part:BBa_K238015:Design

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-09-22)
Revision as of 15:55, 7 October 2009 by AnneV (Talk | contribs) (→‎Design Notes)

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blue light promoter and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757


Design Notes

This part was actually put in a pBR322 vector. This is a vector which has a low copy number (around 15-20). This is needed to make the promoter work properly in E.Coli. The low copy number ensures a good ratio between the promoter and its repressor, which is naturally present in E.Coli. When a high copy number is used, the promoter is abundant which results in a constituitivly active promoter.

Source

the promotor comes from the genome of E.coli MC4100

References