Composite

Part:BBa_K4444015

Designed by: Adiva Daniar   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 04:10, 11 October 2022 by Registry (Talk | contribs)

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PhoCl Secrete Luciferase

Renillla Luciferase comes from a firefly and is a protein that produces fluorescence when it oxidizes its substrate. We assayed the media containing the luciferase using the Promega Luciferase Assay System to analyze the fluorescence of the media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 991
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604
    Illegal BsaI.rc site found at 1684


[edit]
Categories
Parameters
None