Composite
Part:BBa_K4335021
Designed by: Hang Zhang Group: iGEM22_UESTC-BioTech (2022-10-10)
pCrU6.3+insert site+SpScaffold+polyT Term
BBa_ K4335021 consists of a terminator, a cloning site, SpScaffold and a terminator
PCrU6.3 is a previously characterized RNA from chromosome 8 of Chlamydomonas reinhardtii [1] sgRNA
Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'ACTT' on the 5'-terminal of your guide sequence and 'AAAC' on the reverse 5'-terminal.
Usage
pCrU6.3+insert site+SpScaffold+polyT Term is one of the core functional components of plasmid pTX2038 and pTX2040 Its main function is to express specific sgRNA, and combine with HSP70A Promoter+3×Flag+NLS+SpCas9+NLS+Rbcs2 Term(BBa_K4335020)works together to cut specific genes in the nuclear genome of Chlamydomonas reinhardtii, so as to knock out negative lipid related regulatory genes. Sequence and Features
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Categories
Parameters
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