Composite

Part:BBa_K4335021

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)
Revision as of 03:45, 11 October 2022 by ZXFeng (Talk | contribs)


pCrU6.3+insert site+SpScaffold+polyT Term

BBa_ K4335021 consists of a terminator, a cloning site, SpScaffold and a terminator PCrU6.3 is a previously characterized RNA from chromosome 8 of Chlamydomonas reinhardtii [1]

Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'ACTT' on the 5'-terminal of your guide sequence and 'AAAC' on the reverse 5'-terminal.

sgRNA

Sequence and Features BBa_K4335021 SequenceAndFeatures

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Parameters
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