Part:BBa_K4169028
HepT: a RNase like toxin
HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.
Usage and Biology
As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 103
Illegal PstI site found at 127 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 103
Illegal PstI site found at 127 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 103
Illegal PstI site found at 127 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 103
Illegal PstI site found at 127 - 1000COMPATIBLE WITH RFC[1000]
References
Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.
Pcyd: A promoter open in aerobic conditions
This is an aerobically initiated promoter that initiates transcription of subsequent sequences under microaerobic and aerobic conditions. The mechanism is based on the fact that Fnr takes over the promoter sequence under anaerobic conditions, competes with RNA polymerase for binding sites, and thus prevents the transcription of subsequent sequences. However, under microaerobic and aerobic conditions, Fnr protein does not bind to the sequence, and thus can continue the subsequent transcription.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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