Composite

Part:BBa_K4444003

Designed by: June Ahn, Moses Zeidan   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 03:11, 11 October 2022 by Registry (Talk | contribs)

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PhoCl Shed GFP

This backbone contains the plasmid encoding RELEASE, with the furin cut site, HCV cut site, and ER retention motif removed. We inserted PhoCl into the previous furin cut site location. The plasmid contains ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1294


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Categories
Parameters
None