Reporter

Part:BBa_K4245135

Designed by: Bria S VarnBuhler, Jared Moon, Sourav Kumar Dey, Jiahui Wu, Samie R Jaffrey, Akshaya Poonepalle, Shivaek Venkateswaran, Varnica Basavaraj, Manaswi Gorle, Sahana Ram Narayanan, Daeun Lee, Janet Standev   Group: iGEM22_Lambert_GA   (2022-09-23)
Revision as of 01:38, 11 October 2022 by Daeunlee (Talk | contribs)


Lettuce Right

This part is the right component of the split fluorescent DNA aptamer Lettuce BBa_K4245133, developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left BBa_K4245134, induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).


References

VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None