Part:BBa_K4245133:Experience

The Lettuce DNA Aptamer has been tested with DFHBI-1T. The Method adopted by researchers at the Jaffrey Lab was used, and the fluorescence was read on the plate reader. (see Fig. 1)

Figure 1. Graph of fluorescence after DFHBI-1T was added.

The fluorescence of the reaction between the Lettuce aptamer and DFHBI-1T, as seen in Figure 1, appear to be significantly greater than the controls, which contained water, dye, and buffer, as used in the original research. This proved the functionality of the Lettuce DNA fluorescent aptamer, so Lambert iGEM continued with the design and use of the split Lettuce aptamer to better fit the reporting mechanism used in conjunction with Rolling Circle Amplification (RCA).

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