Part:BBa_J428351:Experience

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Cambridge 2022 JUMP DV Characterisation

Experimental approach

JUMP DV Considerations

Out of the 3 JUMP DVs that we characterised in E. coli, we aim to focus on pSC101 as its growth, compared to the other 2 DVs, was less sporadic and didn't go through such a dramatic death phase, instead remaining at the stationary phase until the end of our experiment. Low copy number plasmids are also often used as a default as they exert less burden on cells so this allows us to made a larger contribution to the iGEM and synthetic biologist community. pSC101 being a low-copy number plasmid, will therefore exert less burden on our cells - this is in particular important for the construction and functionality of the complex 'robust perfect adaptation' circuit.

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