Coding

Part:BBa_K4260009:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)
Revision as of 21:27, 10 October 2022 by SofiaCN (Talk | contribs) (→‎Source)

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Coding sequence for isoeugenol monooxygenase and transcriptional activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 431
    Illegal XhoI site found at 259
    Illegal XhoI site found at 286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1179
    Illegal NgoMIV site found at 1887
    Illegal NgoMIV site found at 2495
    Illegal AgeI site found at 1711
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1115
    Illegal BsaI.rc site found at 1807


Design Notes

Codon optimization was performed for both coding sequences.

Source

The transcriptional activator (Iem), and the promoter come from Pseudomonas putida Jin1. Isougenol monooxygenase comes from Pseudomonas putida IE27.

References