Coding
Part:BBa_K4260011:Design
Designed by: Claudia Angélica García Alonso Group: iGEM22_TecCEM (2022-10-09)
Coding sequence for Isoeugenol Monooxygenase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 66
Illegal NgoMIV site found at 774
Illegal NgoMIV site found at 1382
Illegal AgeI site found at 598 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
Design Notes
No considerations were necessary.
Source
The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.