Composite

Part:BBa_K209444:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)
Revision as of 01:38, 6 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

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pEF1-SSFYFP-hM2D-ActA(30-612)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1161
    Illegal NheI site found at 3699
    Illegal PstI site found at 346
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 955
    Illegal BamHI site found at 1665
    Illegal BamHI site found at 2536
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal NgoMIV site found at 3567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal SapI.rc site found at 2317


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References