Composite

Part:BBa_K209440:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)
Revision as of 01:35, 6 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

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pEF1-SSFYFP-hM2D-Tuba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2494
    Illegal EcoRI site found at 3077
    Illegal EcoRI site found at 5088
    Illegal PstI site found at 346
    Illegal PstI site found at 2570
    Illegal PstI site found at 2581
    Illegal PstI site found at 4705
    Illegal PstI site found at 5725
    Illegal PstI site found at 5878
    Illegal PstI site found at 6088
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2494
    Illegal EcoRI site found at 3077
    Illegal EcoRI site found at 5088
    Illegal NheI site found at 1161
    Illegal PstI site found at 346
    Illegal PstI site found at 2570
    Illegal PstI site found at 2581
    Illegal PstI site found at 4705
    Illegal PstI site found at 5725
    Illegal PstI site found at 5878
    Illegal PstI site found at 6088
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2494
    Illegal EcoRI site found at 3077
    Illegal EcoRI site found at 5088
    Illegal BglII site found at 128
    Illegal BglII site found at 4989
    Illegal BglII site found at 5240
    Illegal BamHI site found at 955
    Illegal BamHI site found at 1665
    Illegal BamHI site found at 2278
    Illegal BamHI site found at 4355
    Illegal BamHI site found at 6899
    Illegal XhoI site found at 2504
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2494
    Illegal EcoRI site found at 3077
    Illegal EcoRI site found at 5088
    Illegal PstI site found at 346
    Illegal PstI site found at 2570
    Illegal PstI site found at 2581
    Illegal PstI site found at 4705
    Illegal PstI site found at 5725
    Illegal PstI site found at 5878
    Illegal PstI site found at 6088
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2494
    Illegal EcoRI site found at 3077
    Illegal EcoRI site found at 5088
    Illegal PstI site found at 346
    Illegal PstI site found at 2570
    Illegal PstI site found at 2581
    Illegal PstI site found at 4705
    Illegal PstI site found at 5725
    Illegal PstI site found at 5878
    Illegal PstI site found at 6088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 2537
    Illegal BsaI.rc site found at 2777
    Illegal BsaI.rc site found at 3825
    Illegal BsaI.rc site found at 5402
    Illegal SapI site found at 5547
    Illegal SapI site found at 6141
    Illegal SapI site found at 6575
    Illegal SapI.rc site found at 3534
    Illegal SapI.rc site found at 4311
    Illegal SapI.rc site found at 4402
    Illegal SapI.rc site found at 6097


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References