Composite

Part:BBa_K209437:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)
Revision as of 01:34, 6 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

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pEF1-SSFYFP-hM2D-Beta Pix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2688
    Illegal XbaI site found at 4110
    Illegal PstI site found at 346
    Illegal PstI site found at 2843
    Illegal PstI site found at 3109
    Illegal PstI site found at 3430
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1161
    Illegal PstI site found at 346
    Illegal PstI site found at 2843
    Illegal PstI site found at 3109
    Illegal PstI site found at 3430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 955
    Illegal BamHI site found at 1665
    Illegal XhoI site found at 2992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2688
    Illegal XbaI site found at 4110
    Illegal PstI site found at 346
    Illegal PstI site found at 2843
    Illegal PstI site found at 3109
    Illegal PstI site found at 3430
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2688
    Illegal XbaI site found at 4110
    Illegal PstI site found at 346
    Illegal PstI site found at 2843
    Illegal PstI site found at 3109
    Illegal PstI site found at 3430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 2893
    Illegal BsaI.rc site found at 4195
    Illegal SapI.rc site found at 2499


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References