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Part:BBa_K2981006

Designed by: CHEN,JING   Group: iGEM19_NWU-China   (2019-10-02)
Revision as of 19:58, 10 October 2022 by Ahmed Mattar (Talk | contribs)

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Kp-sp, a secreted peptide

The part is a signal peptide from actinomycetes, and E.coli can also be secreted normally. The signal peptide efficiently enabled extracellular protein secretion and also contributed to the active expression of the intracellular recombinant proteins.

Characterization by mathematical modeling by AFCM-Egypt 2022 team

We are using mathematical modeling to detect the increased level of phenylalanine (phe) in phenylketonuria patients in our diagnostic platform. It depends on a whole cell-based biosensor through a cascade of reactions to finally end by formation of β-galactosidase that turns the color into blue once bound to its substrate (X-gal) as mentioned in figure (4) and graph (1).

Modell1.png
Figure (4) represents the cascade of reactions in whole cell-based biosensor model. Modell11.png


Graph(1) illustrates a direct relation between biomarker and beta-galactosidase ,so as the biomarker increases, the released amount of beta-galactosidase increases till it reaches constant value after about 30 time units. Therefore, the maximum amount of the biomarker releases the maximum amount of beta-galactosidase.

Experimental Characterization by AFCM-Egypt 2022

Capture7.png








This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 3. The running part (ordered from IDT) included KP-SP and lacZ alpha.













Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Yanbing Cui, Yiwei Meng, Juan Zhang,etc.Efficient secretory expression of recombinant proteins in Escherichia coli with a novel actinomycete signal peptide[J].Protein Expression and Purification,2017,129(1):69-74.

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