Coding

Part:BBa_K4307003

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-29)
Revision as of 18:23, 10 October 2022 by Zc123456 (Talk | contribs)


Bxb1 integrase

There are two operators in the genome of bacteriophage, called OR (the right operator) and OL (the left operator) respectively. OR plays an important role in the regulation of gene expression of phage. OR composed of three parts, called OR1, OR2 and OR3. The isolated OR1-OR2 can function as promoter and can be inhibit by cI protein, a repressor expressed by bacteriophage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 180
    Illegal BamHI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 973
    Illegal AgeI site found at 1333
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None