Coding

Part:BBa_K209463:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)
Revision as of 22:59, 5 October 2009 by Katja (Talk | contribs) (→‎Source)

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AarI B-C part, HTR2B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1295
    Illegal PstI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1295
    Illegal PstI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1295
    Illegal BglII site found at 1241
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1295
    Illegal PstI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1295
    Illegal PstI site found at 253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 172
    Illegal SapI.rc site found at 1423


Design Notes

Cloned into pCR2.1.

Source

Plasmid obtained from [http://www.cdna.org/G-Protein-Coupled-Receptors-c8.html Missouri S&T cDNA Resource Center].

References