Composite

Part:BBa_K209434:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 22:11, 5 October 2009 by Katja (Talk | contribs) (Design Notes)

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SSFYFP-Rs1.3-ActA(30-612)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 6348
    Illegal PstI site found at 2984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 4590
    Illegal BamHI site found at 4804
    Illegal BamHI site found at 5185
    Illegal XhoI site found at 4117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal NgoMIV site found at 6216
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 4966


Design Notes

Cloned into BBa_K209399.

Source

References