Composite

Part:BBa_K209430:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 22:09, 5 October 2009 by Katja (Talk | contribs) (Design Notes)

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SSFYFP-hM2D-ITSN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6294
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6294
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 3799
    Illegal PstI site found at 2984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6294
    Illegal BglII site found at 2766
    Illegal BglII site found at 6414
    Illegal BamHI site found at 706
    Illegal BamHI site found at 3593
    Illegal BamHI site found at 4303
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6294
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6294
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal NgoMIV site found at 5065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal SapI.rc site found at 116


Design Notes

Cloned into BBa_K209399.

Source

References