Composite

Part:BBa_K209429:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 22:09, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

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SSFYFP-hM2D-Tuba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5132
    Illegal EcoRI site found at 5715
    Illegal EcoRI site found at 7726
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5208
    Illegal PstI site found at 5219
    Illegal PstI site found at 7343
    Illegal PstI site found at 8363
    Illegal PstI site found at 8516
    Illegal PstI site found at 8726
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5132
    Illegal EcoRI site found at 5715
    Illegal EcoRI site found at 7726
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 3799
    Illegal PstI site found at 2984
    Illegal PstI site found at 5208
    Illegal PstI site found at 5219
    Illegal PstI site found at 7343
    Illegal PstI site found at 8363
    Illegal PstI site found at 8516
    Illegal PstI site found at 8726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5132
    Illegal EcoRI site found at 5715
    Illegal EcoRI site found at 7726
    Illegal BglII site found at 2766
    Illegal BglII site found at 7627
    Illegal BglII site found at 7878
    Illegal BamHI site found at 706
    Illegal BamHI site found at 3593
    Illegal BamHI site found at 4303
    Illegal BamHI site found at 4916
    Illegal BamHI site found at 6993
    Illegal BamHI site found at 9537
    Illegal XhoI site found at 5142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5132
    Illegal EcoRI site found at 5715
    Illegal EcoRI site found at 7726
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5208
    Illegal PstI site found at 5219
    Illegal PstI site found at 7343
    Illegal PstI site found at 8363
    Illegal PstI site found at 8516
    Illegal PstI site found at 8726
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5132
    Illegal EcoRI site found at 5715
    Illegal EcoRI site found at 7726
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5208
    Illegal PstI site found at 5219
    Illegal PstI site found at 7343
    Illegal PstI site found at 8363
    Illegal PstI site found at 8516
    Illegal PstI site found at 8726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 5175
    Illegal BsaI.rc site found at 5415
    Illegal BsaI.rc site found at 6463
    Illegal BsaI.rc site found at 8040
    Illegal SapI site found at 8185
    Illegal SapI site found at 8779
    Illegal SapI site found at 9213
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 6172
    Illegal SapI.rc site found at 6949
    Illegal SapI.rc site found at 7040
    Illegal SapI.rc site found at 8735


Design Notes

Cloned into BBa_K209399.

Source

References