Composite

Part:BBa_K4195124

Designed by: Xiaoping Yu   Group: iGEM22_XMU-China   (2022-09-27)
Revision as of 17:07, 10 October 2022 by Rainbow77 (Talk | contribs)


J23100-B0030-array(targeting pirA/B)-B0015-J23106-B0034-aca2-B0015

Biology

This part was designed as an element of type I CRISPR/Cas system, in which the array contains several designed spacers to target pirA/B gene and repeats derived from Vibrio parahaemolyticus FOC_022 (1) that could be recognized and handled by Cas proteins. Some bacteria can not only encode anti-CRISPR protein (Acr) to inactivate the CRISPR/Cas system, but also encode anti-CRISPR-associated protein (Aca), which is the repressor of acr operon. Aca2 is one of the Aca family discovered with various anti-CRISPRs (2). The pathogen of AHPND, the Vibrio parahaemolyticus that can secret toxin PirA and PirB may also harbor acr and aca2 in the genome (3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 362
    Illegal NheI site found at 385
    Illegal NheI site found at 599
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None