Composite

Part:BBa_K209425:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:37, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-hM4D-Vav


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal PstI site found at 5598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 4719
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal PstI site found at 5598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BglII site found at 5264
    Illegal BglII site found at 7220
    Illegal BamHI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal PstI site found at 5598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal PstI site found at 5598
    Illegal NgoMIV site found at 4590
    Illegal NgoMIV site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 6128
    Illegal BsaI.rc site found at 6322
    Illegal BsaI.rc site found at 7136
    Illegal SapI site found at 5025
    Illegal SapI.rc site found at 116


Design Notes

Cloned into pDONR221.

Source

References