Composite

Part:BBa_K209424:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:37, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-Rs1.3-Tuba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5143
    Illegal EcoRI site found at 5726
    Illegal EcoRI site found at 7737
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5219
    Illegal PstI site found at 5230
    Illegal PstI site found at 7354
    Illegal PstI site found at 8374
    Illegal PstI site found at 8527
    Illegal PstI site found at 8737
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5143
    Illegal EcoRI site found at 5726
    Illegal EcoRI site found at 7737
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 2984
    Illegal PstI site found at 5219
    Illegal PstI site found at 5230
    Illegal PstI site found at 7354
    Illegal PstI site found at 8374
    Illegal PstI site found at 8527
    Illegal PstI site found at 8737
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5143
    Illegal EcoRI site found at 5726
    Illegal EcoRI site found at 7737
    Illegal BglII site found at 2766
    Illegal BglII site found at 7638
    Illegal BglII site found at 7889
    Illegal BamHI site found at 706
    Illegal BamHI site found at 4590
    Illegal BamHI site found at 4804
    Illegal BamHI site found at 4927
    Illegal BamHI site found at 7004
    Illegal BamHI site found at 9548
    Illegal XhoI site found at 4117
    Illegal XhoI site found at 5153
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5143
    Illegal EcoRI site found at 5726
    Illegal EcoRI site found at 7737
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5219
    Illegal PstI site found at 5230
    Illegal PstI site found at 7354
    Illegal PstI site found at 8374
    Illegal PstI site found at 8527
    Illegal PstI site found at 8737
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5143
    Illegal EcoRI site found at 5726
    Illegal EcoRI site found at 7737
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5219
    Illegal PstI site found at 5230
    Illegal PstI site found at 7354
    Illegal PstI site found at 8374
    Illegal PstI site found at 8527
    Illegal PstI site found at 8737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 5186
    Illegal BsaI.rc site found at 5426
    Illegal BsaI.rc site found at 6474
    Illegal BsaI.rc site found at 8051
    Illegal SapI site found at 8196
    Illegal SapI site found at 8790
    Illegal SapI site found at 9224
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 6183
    Illegal SapI.rc site found at 6960
    Illegal SapI.rc site found at 7051
    Illegal SapI.rc site found at 8746


Design Notes

Cloned into pDONR221.

Source

References