Composite

Part:BBa_K209423:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:37, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-hM4D-ITSN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6332
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6332
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 4719
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6332
    Illegal BglII site found at 2766
    Illegal BglII site found at 6452
    Illegal BamHI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6332
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6332
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal NgoMIV site found at 4590
    Illegal NgoMIV site found at 5103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal SapI.rc site found at 116


Design Notes

Cloned into pDONR221.

Source

References