Protein_Domain

Part:BBa_K4375009

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-28)
Revision as of 16:17, 10 October 2022 by NYMP (Talk | contribs)


GS linker with BamHI restriction site

A 13 amino acid long GS linker (Glycine-Serine linker) with a BamHI restriction site at its C-terminal.


Usage and Biology

The GS linker is a flexible linker that can connect different protein domains or regions. This stretch is rich in serine and glycine amino acids and thus provides high mobility to the protein regions conjugated to it. GS linkers are usually useful in connecting antibody fragments to achieve better antigen binding or improve protein folding. The BamHI site makes it easy to clone the linker sequence. This makes it convenient for usage in molecular cloning.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Chen X, Zaro JL, Shen WC. Fusion protein linkers: property, design, and functionality. Advanced drug delivery reviews. 2013 Oct 15;65(10):1357-69.


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