Translational_Unit

Part:BBa_K4187019

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-07)
Revision as of 15:41, 10 October 2022 by LoPezous (Talk | contribs)

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T7-RBS-PHAC1-T1-T7TE

PHAC1 is a lactate-polymerizing Polyhydroxyalkanoate (PHA) synthase that turns the D-lactyl-CoA into polylactate. This is a mandatory intermediate for the polymerization of lactate into polylactate along with pct (BBa_K4187012) .The coding sequence is codon-optimized

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1112
    Illegal NgoMIV site found at 1391
    Illegal AgeI site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None