Coding
H1A

Part:BBa_K4165000

Designed by: Amr Ali Gouda   Group: iGEM22_CU_Egypt   (2022-09-14)
Revision as of 15:35, 10 October 2022 by M Zaki (Talk | contribs)


HTRA Binding Peptide 1

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.8 and IC50 of 0.9 ± 0.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0 cbeta_deviations = 0, clashscore = 69.77, molprobity = 4.26, ramachandran_favored = 40, ramachandran_outliers = 60, Qmean_4 = -6.45183, Qmean6 = -5.51397

Modeling

                 Figure 1.: 3D Model of H1A peptide Modelled by AlphaFold2.0 displayed in Pyomol

Docking

ΔG= -32.8 

                 Figure 1.: 3D Model of H1A peptide Docked against HTRA1 displayed in Pyomol
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Categories
Parameters
None