Composite

Part:BBa_K4165175

Designed by: Ahmed Sameh   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 15:26, 10 October 2022 by M Zaki (Talk | contribs)


Biobrick His - Tau

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), Tau (BBa_K4165009), and T7 terminator (BBa_K731721).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 333
    Illegal BsaI site found at 1245
    Illegal BsaI.rc site found at 45
    Illegal SapI.rc site found at 519


[edit]
Categories
Parameters
None