Plasmid
Part:BBa_K4182006
Designed by: Dan Tan Group: iGEM22_XJTU-China (2022-10-10)
Circuit of blue light induction regulatory system
We designed the upstream control element of the chimeric VVD-AraC fusion structure and the downstream element to verify the effect of the modified operon. We selected sfGFP as the verification protein to efficiently test the expression of the element. The constructed circuit diagram is shown in the following figure.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3502
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 869
Illegal BamHI site found at 6543 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1230
Illegal AgeI site found at 1070
Illegal AgeI site found at 6378 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6151
Illegal BsaI.rc site found at 3824
Illegal SapI site found at 6360
Illegal SapI.rc site found at 39
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Categories
Parameters
None |