Plasmid

Part:BBa_K4182006

Designed by: Dan Tan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 15:20, 10 October 2022 by Registry (Talk | contribs)

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Circuit of blue light induction regulatory system

We designed the upstream control element of the chimeric VVD-AraC fusion structure and the downstream element to verify the effect of the modified operon. We selected sfGFP as the verification protein to efficiently test the expression of the element. The constructed circuit diagram is shown in the following figure.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 869
    Illegal BamHI site found at 6543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1230
    Illegal AgeI site found at 1070
    Illegal AgeI site found at 6378
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6151
    Illegal BsaI.rc site found at 3824
    Illegal SapI site found at 6360
    Illegal SapI.rc site found at 39


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