Coding

Part:BBa_K4182005

Designed by: Dan Tan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 15:19, 10 October 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


FPPs

We designed the upstream control element of the chimeric VVD-AraC fusion structure and the downstream element to verify the effect of the modified operon. We selected sfGFP as the verification protein to efficiently test the expression of the element. The constructed circuit diagram is shown in the following figure.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 103
    Illegal PstI site found at 283
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 103
    Illegal PstI site found at 283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 161
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 103
    Illegal PstI site found at 283
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 103
    Illegal PstI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None