Composite
Part:BBa_K4218004
Designed by: Wuhui Jiang Group: iGEM22_YiYe-China (2022-10-08)
The dual luciferase reporter system
According to previous studies, we found that exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. This mechanism gives rise to our idea of constructing the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. Our project will help us to evaluate the effectiveness of these sensors in diagnosing MDS patients in the future.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2459
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3661
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Categories
Parameters
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