Part:BBa_K2205000

J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 616
  Illegal AgeI site found at 728
  
• 1000
  COMPATIBLE WITH RFC[1000]

Part Documentation by TU_Dresden 2022 Team

Table of content
Biology Expression Comparison to constitutative promoter Item4

1. Biology
2. Expression
3. Comparison to constitutative promoter
4. Item4

Part was compared to <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>.

Figure 1: A. Lac promoter of <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> part and mRFP1 reporter downstream in presence of inductor (IPTG) and in its absence. B. Fluorescence/absorbance 600nm of mRFP1 of the <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> compared to BBa_K2205000 part with <a href="https://parts.igem.org/Part:BBa_J23100">J23100</a> promoter over 24 hour incubation of bacterial culture.

---