Device

Part:BBa_K4244009

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)
Revision as of 13:37, 10 October 2022 by Soniamecacci (Talk | contribs)


Promoter:Dismed2EnvZ:Terminator

The chimeric two-component system (TCS) is formed by combining the extracellular Dismed2 domain of the RetS protein from P. aeruginosa and intracellular HisKA and HATPase intracellular domains of EnvZ part of the EnvZ-OmpR TCS. In this system mucin activates the chimeric TCS, which in turns allow the expression of the essential gene gmk.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
    Illegal NgoMIV site found at 130
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 596
    Illegal NgoMIV site found at 1063
    Illegal NgoMIV site found at 1103
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None