Coding

Part:BBa_K4461005:Design

Designed by: CHIH-YU, YEH   Group: iGEM22_NYCU-Taipei   (2022-10-08)
Revision as of 13:18, 10 October 2022 by YU-TANG-CHANG (Talk | contribs) (→‎References)

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rmf promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment that include the promoter, then use another pair of primers to specify clone of the promoter. Therefore we can avoid the length of the unintended bands being too similar to distinct easily. The sequence we cloned has to include all transcriptional factors for the promoter. We use BBa_B0034 as the RBS of the promoter, so we didn't clone the RBS from the genomic DNA.



Source

The rmf promoter is cloned from E.coli K-12 MG1655 genomic DNA.


References

[1]Smith A, Kaczmar A, Bamford RA, Smith C, Frustaci S, Kovacs-Simon A, O'Neill P, Moore K, Paszkiewicz K, Titball RW, Pagliara S. The Culture Environment Influences Both Gene Regulation and Phenotypic Heterogeneity in Escherichia coli. Front Microbiol. 2018 Aug 15;9:1739. doi: 10.3389/fmicb.2018.01739. PMID: 30158905; PMCID: PMC6104134.