Coding
Part:BBa_K4156126
Designed by: Zheng Huang Group: iGEM22_LZU-CHINA (2022-10-10)
HlpA with INP tag
HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 154
Illegal NgoMIV site found at 405
Illegal AgeI site found at 733 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |