Part:BBa_K4165248

Clamp [6]

Clamp [6] composed of Seed Binding Peptide Seed ( BBa_K4165007) , linker length of GGSGGG (BBa_K4165224), R8 Peptide (BBa_K4165180). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.

Usage and Biology

According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta high enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 35
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

3D Modeling and Ranking

TRRosetta models this composite part with a score of 6 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs).

                        Figure 1. The 3D structure of Clamp [6] modeled by TRrosetta