Composite

Part:BBa_K4343130

Designed by: Zheng Zhe   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 11:52, 10 October 2022 by CSWCMrJi (Talk | contribs)


TEFin-nSpRYCBE

A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nSpRY. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. More critically, SpRY is a PAM-less Cas protein that can be targeted and edited at any location within a gene. At the same time, it is ensured that the editing efficiency is high enough to obtain mutants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1538
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4865
    Illegal BamHI site found at 3817
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 429
    Illegal BsaI site found at 5443
    Illegal BsaI.rc site found at 423
    Illegal BsaI.rc site found at 4552
    Illegal BsaI.rc site found at 5707


[edit]
Categories
Parameters
None