Coding

Part:BBa_K4461001:Design

Designed by: YUAN, HSIAO   Group: iGEM22_NYCU-Taipei   (2022-10-08)
Revision as of 11:49, 10 October 2022 by YU-TANG-CHANG (Talk | contribs) (→‎References)

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cyoA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment that include the promoter, then use another pair of primers to specify clone of the promoter. Therefore we can avoid the length of the unintended bands being too similar to distinct easily. The sequence we cloned has to include all transcriptional factors for the promoter. We use BBa_B0034 as the RBS of the promoter, so we didn't clone the RBS from the genomic DNA.



Source

The cyoA promoter is cloned from E.coli K-12 MG1655 genomic DNA.


References

[1]Smith A, Kaczmar A, Bamford RA, Smith C, Frustaci S, Kovacs-Simon A, O'Neill P, Moore K, Paszkiewicz K, Titball RW, Pagliara S. The Culture Environment Influences Both Gene Regulation and Phenotypic Heterogeneity in Escherichia coli. Front Microbiol. 2018 Aug 15;9:1739. doi: 10.3389/fmicb.2018.01739. PMID: 30158905; PMCID: PMC6104134.

[2]Cotter PA, Gunsalus RP. Contribution of the fnr and arcA gene products in coordinate regulation of cytochrome o and d oxidase (cyoABCDE and cydAB) genes in Escherichia coli. FEMS Microbiol Lett. 1992 Feb 1;70(1):31-6. doi: 10.1016/0378-1097(92)90558-6. PMID: 1315704.

[3]Bradley MD, Beach MB, de Koning APJ, Pratt TS, Osuna R. Effects of Fis on Escherichia coli gene expression during different growth stages. Microbiology (Reading). 2007 Sep;153(Pt 9):2922-2940. doi: 10.1099/mic.0.2007/008565-0. PMID: 17768236.

[4]Hommais F, Krin E, Coppée JY, Lacroix C, Yeramian E, Danchin A, Bertin P. GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli. Microbiology (Reading). 2004 Jan;150(Pt 1):61-72. doi: 10.1099/mic.0.26659-0. PMID: 14702398.