Tag

Part:BBa_K4165070

Designed by: Ahmed Sameh   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 10:25, 10 October 2022 by Esraa Elmligy (Talk | contribs)


Glutathione S-Transferase

This Part encodes for the GST tag which serve as an enhancing tag and also serves in purification of expressed proteins in bacterial expression system.

Usage and Biology

Glutathione S-transferase (GST), used for affinity purification when fused to a gene of interest [1]. Glutathione-agarose affinity chromatography provides the quick, delicate, non-denaturing, and very selective purification of glutathione-binding sequence-containing proteins, such as Glutathione S-Transferase (GST), glutathione peroxidase, and glyoxalase. (DocS and Coh are fused with both GST and His tag to determine which one will give more yield and stability)


References

1- Harper, S., & Speicher, D. W. (2011). Purification of proteins fused to glutathione S-tranferase. Methods in molecular biology (Clifton, N.J.), 681, 259. https://doi.org/10.1007/978-1-60761-913-0_14.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None