Reporter

Part:BBa_K215010

Designed by: Jeff Nivala   Group: iGEM09_Washington   (2009-10-01)
Revision as of 18:39, 1 October 2009 by Swanson (Talk | contribs)

pLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP

This part is GFP with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BioBrick BBa_K215107). Its expression is IPTG inducible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 842


[edit]
Categories
Parameters
n/apLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP