Part:BBa_K4375017
anti-CEA Nanobody Display System
This construction consists of 4 described parts. These are: J23101* (BBa_K4375003), anti-CEA-specific Nanobody, (BBa_K4375006 ), Intimin (BBa_K4375012), Myc (BBa_K4375014). They allow the bacterium to bind to the cancer cell surface, thus allowing its visualization and colonization on the tumor surface.
Usage and Biology
This construct is meant for detecting cancer cells that express CEA, carcioembrional antigen by bacterial cells. It is expressed by the bacterial cells on their surface and can be used to detect the tumor by as it causes the bacteria to bind to cancer cells. This device contains two different parts whose production is controlled by a constitutive strong promoter J23101* (BBa_K4375003). The anti-CEA Nanobody (BBa_K4375006) is at the N-terminal, tagged with a c-myc for detection. The Nanobody is fused to the Intimin part, which is a 12-stranded β-barrel protein found in bacterial adhesins (BBa_K4375012). Specific interaction can be detected by two tags: Myc (BBa_K4375014) and anti-E, or via the fluorescence of the sfGFP at the C-terminal.
Intimin and CEA nanobody conjugation scheme.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 29 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2043
Illegal BamHI site found at 4059 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2064
Illegal NgoMIV site found at 4080
Illegal AgeI site found at 4146
Illegal AgeI site found at 4278
Illegal AgeI site found at 4678 - 1000COMPATIBLE WITH RFC[1000]
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