Part:BBa_K4241792

mCherry XL RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Overview

mCherry-XL is a basic red fluorescent protein published in 2022, derived from Discosoma sp.. This RFP is evolved to have increased lifespan, increased quantum yield to be on par as the brightest RFP.

Usage and Biology

mCherry-XL is derived from a lifetime-based evolution of mCherry. Four positions were identified near the FP chromophore to be mutated to increase lifetime, resulting in the derivative mCherry-XL (eXtended Lifetime). This RFP boasts an impressive 3-fold quantum yield matching the brightest RFP to date mScarlet.
This is a gene to be used as a reporter. This can be done via fluorescent microscopy.

References

Mukherjee, S., Manna, P., Hung, S.-T., Vietmeyer, F., Friis, P., Palmer, A., & Jimenez, R. (2022). Directed evolution of a bright variant of mcherry: Suppression of non-radiative decay by fluorescence lifetime selections. https://doi.org/10.26434/chemrxiv-2022-v3t03