Part:BBa_K4335013

SpCas9

The Cas9 protein is part of the bacteria's CRISPR/Cas9 immune defense mechanism to identify and destroy foreign DNA. By incorporating the foreign DNA into the bacteria's own DNA, it has a memory of any prior foreign DNA that the bacteria has encountered.The Cas9 endonuclease can be used either with the tracrRNA and crRNA (CRISPR RNA- Clustered Regularly Interspaced Short Palindromic Repeats) or with a guide RNA to cleave foreign DNA at a species locations. This makes exceptionally useful for DNA cloning in synthetic biology.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 241
  Illegal PstI site found at 787
  Illegal PstI site found at 2209
  Illegal PstI site found at 2413
  Illegal PstI site found at 2443
  Illegal PstI site found at 3655
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 241
  Illegal PstI site found at 787
  Illegal PstI site found at 2209
  Illegal PstI site found at 2413
  Illegal PstI site found at 2443
  Illegal PstI site found at 3655
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 248
  Illegal BglII site found at 1043
  Illegal BglII site found at 1733
  Illegal BglII site found at 3812
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 241
  Illegal PstI site found at 787
  Illegal PstI site found at 2209
  Illegal PstI site found at 2413
  Illegal PstI site found at 2443
  Illegal PstI site found at 3655
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 241
  Illegal PstI site found at 787
  Illegal PstI site found at 2209
  Illegal PstI site found at 2413
  Illegal PstI site found at 2443
  Illegal PstI site found at 3655
  Illegal NgoMIV site found at 1075
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3468