Part:BBa_K4361017

BlcR-binding oligo, 106 bp, IR1+IR2 + 15 bp linker + IR1+IR2

BlcR is a transcription factor originating from the bacterium Agrobacterium tumefaciens (Part:BBa_K4361100). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs (IRs), short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' (IR1) and 'ATTAGttgaactCTAAT' (IR2), as further explained in Part:BBa_K4361001.
This part and Part:BBa_K4361019 have been designed to test whether or not multiple separate BlcR tetramers can form on a single DNA molecule. To create this part, a second IR1-tca-IR2 set has been added after the 3' end of the original IR2 in the wildtype sequence, spaced by a 15 nt linker (gcgggtcagcgggtc). The BlcR-binding domain of this part thus consists of IR1-tca-IR2-gcgggtcagcgggtc-IR1-tca-IR2. The distance between the IR sets is assumed to be sufficient to prevent two potential BlcR tetramers from sterically hindering each other, as well as placing them on opposite sides of the DNA molecule due to being spaced 15 nt apart.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]