Coding
fiatluxB

Part:BBa_K4239004:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-06)
Revision as of 07:41, 10 October 2022 by Gfulconis (Talk | contribs) (→‎Design Notes)


Enhanced luciferase substrate forming unit fiatluxB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fiatluxB did not have any mutation compare to iluxB, because no igem restriction site (EcoR1, Xba1, Spe1 and Pst1) were into the gene.

iluxB had mutations from luxB (S13P, V121A, N259D). They changed the protein sequence, in order to create a system that produced more light. The first and the last letters correspond to the Amino Acid before and after mutation, and the number indicates its position in the protein. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018.

Source

aze

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.