RNA

Part:BBa_K4169005

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 05:18, 10 October 2022 by Sijia-Xu (Talk | contribs)

BBa_K4169005

RNA Thermometer

Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733011) was used in this study.

Usage and Biology

The ScGS is a bifunctional sesquiterpene cyclase, with the presence of Mg2+, the N-terminal half of this protein catalyzes the ionization and cyclization of farnesyl diphosphate to form germacradienol and inorganic pyrophosphate(PPi). Then the C-terminal domain, highly homologous with the former, catalyzes the protonation, cyclization, and fragmentation of germacradienol to form geosmin and acetone.1111


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 39


References

Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.

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Categories
Parameters
biologyEscherichia coli